Small highly active dnas that hydrolyze dna

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August undefined, 2024

Webb5 juni 2013 · Natural DNA sequences conforming to the class I consensus sequence and structure were found that undergo hydrolysis under selection conditions (2 mM Zn(2+), pH 7), which demonstrates that the inherent structure of certain DNA regions might promote catalytic reactions, leading to genomic instability. Menu Log inSign up Search Papers Webb10 jan. 2024 · Developing nanomaterial-based enzyme mimics for DNA cleavage is an interesting challenge and it has many potential applications. Single-layered graphene …

Small, Highly Active DNAs That Hydrolyze DNA - figshare.com

Webb6 juni 2013 · Natural DNA sequences conforming to the class I consensus sequence and structure were found that undergo hydrolysis under selection conditions (2 mM Zn(2+), … WebbSmall, highly active DNAs that hydrolyze DNA. Article. Full-text available. May 2013; J AM CHEM SOC; Hongzhou Gu; Kazuhiro Furukawa; Zasha Weinberg; Daniel F Berenson; Ronald R Breaker; how to remove shading behind text in word

DNAmoreDB - A Database of Deoxyribozymes - GeneSilico

Webbför 2 dagar sedan · Herein, based on the mobility change of DNA in denaturing gels, we develop a selection strategy to discover a series of 40-45 nt small DNAs that can bind … WebbLigation of linear single-stranded DNAs with CircLigase to form circles. CircLigase is a thermostable ATP-dependent single-stranded DNA ligase that catalyzes intramolecular ligation of single-stranded DNA templates. The ligation substrates must carry 5′-phosphate a 3′-hydroxyl groups on the donor and acceptor DNAs, respectively. Webb4 nov. 2024 · Small DNAs that Bind Nickel (II) Specifically and Tightly. Metal recognition by nucleic acids provides an intriguing route for biosensing of metal. Toward this goal, a … normal schirmer test values

Small DNAs that Bind Nickel(II) Specifically and Tightly.

DNAmoreDB - A Database of Deoxyribozymes

Webb(B) Hydrolytic instability of natural DNA sequences. Trace amounts of each natural DNA (5′ 32P-labeled) were incubated at 37 °C in reaction buffer without or with 2 mM Zn2+ for 6 … Webb6 okt. 2024 · Herein, we propose a DNAzyme-guided DNA amplification motor through the facile built-in of a stimuli-responsive DNAzyme brake to facilitate the in vivo sensitive imaging of biomarkers by minimizing the off-site signal leakage in living systems. how to remove shading from pdfWebbMembers of the single-stranded DNA pool contain two 15 nt primer binding sites (5′ and 3′ termini) and two 50 nt random-sequence domains ... Reported in... Year of Publication First Author Laboratory Title PubMed ID DOI Reaction; 2013: H Gu: R R Breaker: Small, highly active DNAs that hydrolyze DNA. 23679108: 10.1021/ja403585e: DNA cleavage ... how to remove shading in google docs

"WebbNatural DNA sequences conforming to the class I consensus sequence and structure were found that undergo hydrolysis under selection conditions (2 mM Zn 2+, pH 7), which … " - Small highly active dnas that hydrolyze dna

Small highly active dnas that hydrolyze dna

Functional Compromises among pH Tolerance, Site Specificity, …

Webb6 okt. 2010 · Small, Highly Active DNAs That Hydrolyze DNA. Journal of the American Chemical Society 2013, 135 (24) , 9121-9129. DOI: 10.1021/ja403585e. Darren J. Parker, Ying Xiao, John M. Aguilar, and Scott K. Silverman . DNA Catalysis of a Normally Disfavored RNA Hydrolysis ...

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WebbNucleotides in black are present in the parent construct used for reselection, but these positions are not well conserved in the selected population. Arrows designate the site of … Webb14 okt. 2024 · Catalytic DNAs, mainly DNA/RNA-cleaving DNAs, ... with the total 17 conserved nucleotides forming a catalytic loop to site-specifically hydrolyze DNA in the loop upon the supplementation of Zn 2+ ... Small, highly active DNAs that hydrolyze DNA. J. Am. Chem. Soc., 135 (2013), pp. 9121-9129.

Webb1 juni 2013 · I-R3 DNAzyme is a small, highly active catalytic DNA for DNA hydrolysis. In here, we designed two cis-structure DNAzymes (I-R3N and I-R3S) based on the different locates of the joint linker ... Webb14 okt. 2024 · DNA nanosponges are an emerging attractive type of nanocarriers for targeted therapeutics due to their ability to integrate diverse molecules, including DNA …

WebbDNA phosphoester bonds are exceedingly resistant to hydrolysis in the absence of chemical or enzymatic catalysts. This property is particularly important for organisms … Webb6 juni 2013 · DNA phosphoester bonds are exceedingly resistant to hydrolysis in the absence of chemical or enzymatic catalysts. This property is particularly important for …

WebbJournal of the American Chemical Society, 135 (24), 9121–9129 10.1021/ja403585e. Gu, H., Furukawa, K., Weinberg, Z., Berenson, D. F., & Breaker, R. R. (2013). Small, Highly …

Webb6 okt. 2024 · Flow cytometry analysis. The MCF-7 cells (human breast tumor cell line), HeLa cells (cervical tumor cell line), and MCF-10A cells (human breast normal cell line) were seeded at a density of 2 × 10 5 per well onto a 6-well plate and cultured. When the cells reached 90% confluency, the F-ZD nanoparticles dispersed in the Opti-minimum … how to remove shading in word documentWebb6 juni 2013 · The identification of small DNA-cleaving deoxyribozymes that function with high speed under mild reaction conditions should permit their use in various … how to remove shading in microsoft wordWebb27 jan. 2011 · Small, highly active DNAs that hydrolyze DNA. Hongzhou Gu, K. Furukawa, Z. Weinberg, Daniel F. Berenson, R. Breaker; Biology. ... Using in vitro selection, this work has identified deoxyribozymes that sequence-specifically hydrolyze DNA with multiple turnover and rate enhancement of 108 ... how to remove shading in word tableWebbSmall, Highly Active DNAs That Hydrolyze DNA: 23679108: 10.1021/ja403585e: 2013: Liu, Y. He, J. Breaking the conservation of guanine residues in the catalytic loop of 10–23 DNAzyme by position-specific nucleobase modifications for rate enhancement: 23615852: 10.1039/c3cc42067a: 2024: Lee, Y. Silverman, S. K normal science and post normal scienceWebbDNA cleavage by 10MD5 proceeds with kobs = 2.7 h −1 and rate enhancement of 10 12 over the uncatalyzed P−O hydrolysis reaction. 10MD5 has a very sharp pH optimum near 7.5, with greatly reduced DNA cleavage rate and yield when the pH is changed by only 0.1 unit in either direction. normal school literary magazineWebbDNA, Catalytic "DNA, Catalytic" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings). Descriptors are arranged in a hierarchical structure, which enables … normal school leaving age ukWebbThe development of a simple DNA sensor based on DNAzyme and PCR and aimed at the detection of low concentrations of zinc (II) ions, which showed sensitivity (10 nM) and high specificity for zinc ion detection is described. AbstractThe development of a simple sensor (9NL27-Zn) based on DNAzyme and PCR and aimed at the detection of low … normal schools apush definition